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Mary-Sue Brown was the final Associate of the Third Inspector and the first Associate of the Fourth. She was played by Mary McDonnell during Seasons 11-14. Initially introduced to the show as a feminist photographer, she begins traveling with the Inspector after sneaking onto his DARSIT. During her travels, she met the Blogons, Arachnids, Rostraaans, Metalguys, and Squid-beings. When McDonnell chose to leave the series during Season 14, it made front-page news, something that had never before happened to an associate. She is forced to leave the Inspector in the episode "The Foot of Terror" after he is called back to Kayaclasch. Although it is never explicitly stated, it is heavily implied that Mary-Sue had feelings for the Inspector when she traveled with him as an Associate. In the reboot of the series, she was reunited with the Tenth Inspector in the Series Two episode "The Embarrassing 15th Anniversary Reunion". She also reappeared in the Series Four episodes "Return to Earth" and "Finale" and at the end of the 2009 Christmas Special "The Last Minutes" in a non-speaking role. Given her role as the Fourth Inspector's Associate and that Inspector's rating as the Most Popular Inspector by the public, she has consistently been rated Most Popular Associate, though Lily Taylor and Constable Horace Wigglesworth have taken over that spot in recent years. Mary-Sue was intended to be the star of an Inspector Spacetime spinoff known as FE-Line and Friends, but it never got off the ground. The character was given her own children's spinoff in 2006, The Mary-Sue Mysteries, where she defends Earth from alien invasions with help from her adopted son Mark, FE-Line, and their teenage friends Elizabeth, Nitya, and Roy.

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